Application

Protein G′ from Streptococcus sp. has been used in in vitro actin labeling assay.

Biochem/physiol Actions

Protein G can bind all the human and mouse IgG subclasses. It can bind to both the fragment crystallizable (Fc) and antigen-binding fragment (Fab) components of the antibody.

General description

Protein G, a cell wall protein, is obtained from group G streptococci. The extracellular part of this protein is made of two/three small domains or serum albumin binding (GA domains) and two/three immunoglobulin (IgG) binding domains (B domains).

Genetically engineered truncated protein G; retains affinity for IgG, but lacks albumin and Fab binding sites and membrane-binding regions.

Physical form

lyophilized powder in a Tris-HCl buffer, pH 7.5